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Description
The i CapTag TM resin is designed to capture recombinant proteins tagged with the i CapTag TM , a self-cleaving affinity tag for the rapid and simple purification of tagless proteins.
Prepacked i CapTag TM columns are designed for simple purification of tagless proteins via a self-cleaving affinity tag.Product Specifications
Table 1. Characteristics of the i CapTag TM affinity chromatography.
Parameters |
Requirements* |
Packing size | 1 x 5 mL |
Bed dimensions | 8 x 100 mm |
Bed volume | 5 mL |
Flow rate** | Recommended flow rate is 1.25 mL/min (see manual for more details). Maximum flow rate is 2 mL/min. |
Ligand Density |
15 ± 2 g/L |
Cleavability & |
95% or higher cleavage in 5 hours at room temperature (based on GFP test target protein) |
Base Bead |
Hardened agarose (6% cross-linked agarose) |
Particle Size, d 50v |
~90 μm |
Maximum Back Pressure |
40 psi (2.8 bar; 0.28 MPa) |
Typical Working Temperature # |
|
Chemical Stability |
Resin is initially stripped at pH ≤ 2.0 followed by buffers with the pH between 6.2 and 8.5. Resin is neutral to salts, sugars and other buffer additives, including protease inhibitors and stays active when caustic buffers are used. |
Resin Packing |
50% slurry in storage solvent in 18% ± 2% ethanol |
Storage |
18% ± 2% ethanol, 4 ℃ to 8 ℃ |
Abbreviations & Notes
* - Specific parameters of the resin are included in the Certificate of Analysis based on the lot number of the resin.
** - The pressure varies based on column tubing and chromatography medium.
& - Note that the first two amino acids of protein of interest have a significant impact on the cleavage rate, where aromatic residues tend to accelerate cleaving while smaller residues slow or stop cleaving and proline abolishes cleaving (see additional instructions). For proteins with unacceptably slow cleaving, amino acids can be added or modified at the N-terminus to provide more optimal cleaving.
# - Note that the temperature effects cleaving. Typically, lower temperatures slow down cleaving rate and higher speed up cleavage.
d 50v - Median particle size of the cumulative volume distribution.
Safety Information
Ethanol, solution: 18% ± 2%
Hazard class: 3
Identification number: UN1170
Packing Groups: PG 3
Download product manual for rapid implementation

Download examples of DNA plasmids containing target proteins
Download & Print MSDS
FPLC Columns
Download & Print MSDS
Bulk Resin
Download the Certificate of Analysis
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Protein Capture Science team would like to thank federal governmental agencies and private companies for supporting development of this innovative technology and many previous prototype iterations, which cumulatively led to iCapTag platform development. Many thanks go to wonderful teams supporting commercialization of iCapTag technology during the COVID-19 pandemic and grant agencies, including State of Ohio, Ohio Third Frontier, Ohio Development Services Agency, Rev1 Ventures, the Ohio State University, extraordinary collaborators and vendors.
This Product is a Result of Many Years of Research and Development Thanks to Governmental Grants Awards Supporting
Dr. David Wood Laboratory at Princeton University and the Ohio State University.
All Rights Reserved | Protein Capture Science