Description

The i CapMag ^TM magnetic beads is designed to capture recombinant proteins tagged with the i CapMag ^TM , a self-cleaving affinity tag for the rapid and simple purification of tagless proteins. Additional ranges of product volumes will be available soon. Free shipping is available for iCapMag products with total sale of $300 and over.

Prepacked i CapMag ^TM columns are designed for simple purification of tagless proteins via a self-cleaving affinity tag.

• The i CapMag ^TM magnetic beads binds with high specificity to the i CapMag ^TM , a 36-amino acid sequence appended to the N-terminus of the target protein.
• Strong and selective binding at pH 8.5 allows purification of the target protein via a conventional affinity purification approach.
• Once purified, the i CapMag ^TM is induced to release the target protein from the bound tag via a change in buffer pH from 8.5 to 6.2.
• Controllable cleavage to 90% released target typically takes place in less than 30 minutes to 1 hour at room temperature (depending on the N-terminal amino acids of the target protein). Prescribed modifications of the first two amino acids of the target can be used to optimize cleaving. In some cases, cleaving could be performed overnight as well.
• A wide range of temperatures can be used during the purification process including 36 ℃ , room temperature or cold conditions (e.g., 4 ℃ ).
• Once the released target protein is recovered in the column effluent, the i CapMag ^TM magnetic beads can be stripped at reused by a low pH or higher pH wash.
• The i CapMag ^TM magnetic beads has been used successfully for proteins expressed in E. coli , CHO and HEK cell lines, as well as cell-free systems and non-standard host cells.

Product Specifications

Table 1. Characteristics of the i CapMag ^TM magnetic beads for proteins and peptides purification.

Parameters	Requirements*
Packing size	1 x 100 microL in 900 microL of 18 ± 2% ethanol
Bed volume	100 microL
Particle Size	20-40 µm (30 µm average)
Base Bead	Spherical magnetic cross-linked 6% agarose beads
Ligand Density	20 ± 2 mg per 1 mL
Cleavability &	95% or higher cleavage was observed as early as 10 min at 37 ℃ (based on GFP test target protein, although it is protein/peptide dependent)
Typical Working Temperature #	4 ℃ to 37 ℃
Chemical Stability	M agnetic beads are initially stripped followed by buffers with the pH between 6.2 and 8.5. Beads are neutral to salts, sugars and other buffer additives, including protease inhibitors and stays active when caustic buffers are used.
Magnetic Beads Storage and Packing	Magnetic beads are immersed in storage solvent (18% ± 2% ethanol)
Storage	18% ± 2% ethanol, 4 ℃ to 8 ℃
Design by	Protein Capture Science (made in USA)
Contact	support@proteincapturescience.com
Usage	Research Use Only

Abbreviations & Notes

* - Specific parameters of the product are included in the Certificate of Analysis based on the lot number of the product.

& - Note that the first two amino acids of protein of interest have a significant impact on the cleavage rate, where aromatic residues tend to accelerate cleaving while smaller residues slow or stop cleaving and proline abolishes cleaving (see additional instructions). For proteins with unacceptably slow cleaving, amino acids can be added or modified at the N-terminus to provide more optimal cleaving.

# - Note that the temperature effects cleaving. Typically, lower temperatures slow down cleaving rate and higher speed up cleavage.

Safety Information

Ethanol, solution: 18% ± 2%

Hazard class: 3

Identification number: UN1170

Packing Groups: PG 3